Pseudopyricularia

Klaubauf et al., Stud. Mycol. 79: 109. 2014.

Classification: Sordariomycetes, Sordariomycetidae, Magnaporthales, Pyriculariaceae.
Type species: Pseudopyricularia kyllingae Klaubauf et al. Holotype and ex-type culture: CBS H-21841, CBS 133597.
DNA barcodes (genus): LSU, rpb1.
DNA barcodes (species): ITS, rpb1, act, cal.

Sexual morph unknown. Conidiophores solitary, erect, straight or curved, branched or not, medium brown, smooth or finely roughened, septate. Conidiogenous cells integrated, terminal, rarely intercalary, medium brown, smooth or finely roughened, forming a rachis with several protruding denticles usually flat-tipped. Conidia solitary, obclavate, pale to medium brown, smooth or/to finely roughened, guttulate, 1–2-septate; hila truncate, slightly protruding, unthickened, not darkened (adapted from Klaubauf et al. 2014).

Culture characteristics:

Colonies smooth with sparse to moderate aerial mycelium. On MEA transparent, buff, honey to isabelline or white with patches of greyish sepia. On OA transparent sometimes with patches of olivaceous grey or greyish sepia. On PDA transparent, white, greyish sepia or olivaceous black.

Optimal media and cultivation conditions:

Sterile barley seed on SNA at 25 °C under continuous near-ultraviolet light to promote sporulation.

Distribution:

Mainly found in Asia, but also in North America, Africa and New Zealand.

Hosts:

Pathogens of Cyperaceae, but also found on Bothriochloa bladhii (Poaceae) and Typha orientalis (Typhaceae).

Disease symptoms:

Leaf spots.

Notes:

Pseudopyricularia was one of the genera introduced recently in order to resolve the polyphyletic nature of Pyricularia based on a phylogenetic study that included five different loci (Klaubauf et al. 2014). Pseudopyricularia is mainly distinguished from Pyricularia by having short, determinate, brown conidiophores with an apical rachis with flat-tipped denticles.

Reference:

Klaubauf et al. 2014 (morphology and phylogeny).
Klaubauf S, Tharreau D, Fournier E, et al. (2014). Resolving the polyphyletic nature of Pyricularia (Pyriculariaceae). Studies in Mycology 79: 85–120.

Table 17. DNA barcodes of accepted Pseudopyricularia spp.

Species	Isolates¹	GenBank accession numbers²				References
		ITS	*rpb1*	*act*	*cal*
Py. bothriochloae	CBS 136427^T	KF777186	KY905701	KY905700	-	Crous et al. (2013), Marin-Felix et al. (2017)
Py. cyperi	CBS 133595^T	KM484872	AB818013	AB274453	AB274485	Klaubauf et al. (2014), Murata et al. (2014), Hirata et al. (2014)
Py. hagahagae	CPC 25635^T	KT950851	KT950877	KT950873	-	Crous et al. (2015)
Py. higginsii	CBS 121934	KM484875	KM485095	KM485180	KM485250	Klaubauf et al. (2014)
Py. kyllingae	CBS 133597^T	KM484876	KM485096	AB274451	AB274484	Klaubauf et al. (2014), Hirata et al. (2014)

¹CBS: Westerdijk Fungal Biodiversity Institute, Utrecht, the Netherlands; CPC: Culture collection of Pedro Crous, housed at Westerdijk Fungal Biodiversity Institute. ^T indicates ex-type strains.

²ITS: internal transcribed spacers and intervening 5.8S nrDNA; rpb1: partial RNA polymerase II largest subunit gene; act: partial actin gene; cal: partial calmodulin gene.

Crous PW, Wingfield MJ, Guarro J, et al. (2013). Fungal Planet description sheets: 154–213. Persoonia 31: 188–296.
Crous PW, Wingfield MJ, le Roux JJ, et al. (2015). Fungal Planet description sheets: 371–399. Persoonia 35: 264–327.
Hirata K, Kusaba M, Chuma I, et al. (2014). Speciation in Pyricularia inferred from multilocus phylogenetic analysis. Mycological Research 111: 799–808.
Klaubauf S, Tharreau D, Fournier E, et al. (2014). Resolving the polyphyletic nature of Pyricularia (Pyriculariaceae). Studies in Mycology 79: 85–120.
Marin-Felix Y, Groenewald JZ, Cai, L, et al. (2017). Genera of phytopathogenic fungi: GOPHY 1. Studies in Mycology xxxx.
Murata N, Aoki T, Kusaba M, et al. (2014). Various species of Pyricularia constitute a robust clade distinct from Magnaporthe salvinii and its relatives in Magnaporthaceae. Journal of General Plant Pathology 80: 66–72.