Crous, et al., Persoonia 34: 201. 2015. Fig. 55.

Fig. 55. Wojnowiciella spp. A–F. Conidiomata overview. A. Wojnowiciella leptocarpi (ex-type CBS 116584). B, E. Wojnowiciella dactylidis (CPC 30353). C. Wojnowiciella cissampeli (ex-type CBS 141297). D. Wojnowiciella eucalypti (ex-type CBS 139904). F. Wojnowiciella dactylidis (CPC 32741). G, H. Hand section of the conidiomata with hyaline conidiogenous cells and dark brown conidia of Wojnowiciella leptocarpi (ex-type CBS 116584). I–L. Conidiogenous cells. I, J. Wojnowiciella cissampeli (ex-type CBS 141297). K. Wojnowiciella eucalypti (ex-type CBS 139904). L. Wojnowiciella leptocarpi (CBS 116585). M–Q. Macroconidia. M, N. Wojnowiciella leptocarpi (ex-type CBS 116584). O. Wojnowiciella cissampeli (ex-type CBS 141297). P. Wojnowiciella dactylidis (CPC 32741). Q. Wojnowiciella dactylidis (CPC 30353). R. Microconidia of Wojnowiciella eucalypti (ex-type CBS 139904). Scale bars: E–G = 50 μm; H = 20 μm; others = 10 μm. Pictures taken from Crous et al., 2015d, Crous et al., 2016b.

Classification: Dothideomycetes, Pleosporomycetidae, Pleosporales, Phaeosphaeriaceae.

Type species: Wojnowiciella eucalypti Crous, et al. Holotype and ex-type strain: CBS H-22233, CBS 139904.

DNA barcode (genus): LSU.

DNA barcodes (species): ITS, rpb2, tef1. Table 21. Fig. 56.

Table 21. DNA barcodes of accepted Wojnowiciella spp.

Species Isolates1 GenBank accession numbers2 References
ITS LSU rpb2 tef1
Wojnowiciella cissampeli CBS 141297T KX228272 KX228323 LT990616 Crous et al. (2016b), present study
W. dactylidis MFLUCC 13-0735T KP744470 KP684149 Liu et al. (2015)
CPC 27468 LT990658 LT990630 LT990644 LT990611 Present study
CPC 30353 LT990659 LT990631 LT990612 Present study
CPC 32741 LT990660 LT990632 LT990613 Present study
CPC 33929 LT990661 LT990633 LT990645 LT990614 Present study
W. eucalypti CBS 139904T KR476741 KR476774 LT990617 Crous et al. (2015d), present study
W. leptocarpi CBS 115684T KX306775 KX306800 LT990646 LT990615 Hernández-Restrepo et al. (2016c), present study
W. lonicerae MFLUCC 13-0737T KP744471 KP684151 Liu et al. (2015)
W. spartii MFLUCC 13-0402T KU058719 KU058729 Li et al. (2015)
W. viburni MFLUCC 12-0733T KC594286 KC594287 Wijayawardene et al. (2013)

CBS: Westerdijk Fungal Biodiversity Institute, Utrecht, the Netherlands; CPC: Personal collection of Pedro Crous housed at the Westerdijk Fungal Biodiversity Institute; MFLUCC: Mae Fah Luang University Culture Collection, Chiang Rai, Thailand. T indicates ex-type strain.


ITS: internal transcribed spacers and intervening 5.8S nrDNA; LSU: partial large subunit RNA gene; rpb2: partial RNA polymerase II second largest subunit gene; tef1: partial translation elongation factor 1-alpha gene.

Fig. 56. RAxML phylogram obtained from the combined ITS (742 bp), LSU (885 bp), rpb2 (1029 bp) and tef1 (998 bp) sequence alignment of all the accepted species of Wojnowiciella. The tree was rooted to Phaeosphaeria caricis CBS 120249 and Septoriella hirta CBS 536.77. RAxML bootstrap support (BS) values above 70 % are shown in the nodes. GenBank accession numbers are indicated in Table 21. T indicates ex-type strains. TreeBASE: S21899.

Conidiomata pycnidial, globose, brown, separate, non-papillate or papillate, with central ostiole; conidiomatal wall composed of 3–6 layers of brown cells, textura angularis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells lining cavity, hyaline to pale brown, smooth, ampulliform to subcylindrical, appearing phialidic. Macroconidia subcylindrical, straight to slightly curved, apex subobtuse, base truncate, septate, at times with 1–2 oblique septa, thick-walled, verruculose, guttulate, golden brown. Microconidia in same or different conidiomata as macroconidia. Microconidiophores intermingled with macroconidiogenous cells, branched at base, septate, subcylindrical, hyaline, smooth. Microconidiogenous cells terminal and intercalary, hyaline, smooth, ampulliform to subcylindrical, phialidic with periclinal thickening. Microconidia solitary, hyaline, guttulate, smooth, subcylindrical to ellipsoid, apex obtuse to subobtuse, base truncate.

Culture characteristics: Colonies on MEA, cottony, isabelline, greenish olivaceous, mouse grey to greyish sepia, sometimes with luteous exudate; reverse greyish sepia, chestnut, fulvous. Colonies on PDA pale mouse grey, brown vinaceous or greenish olivaceous, sometimes with luteous diffusible pigment; reverse luteous and black, greyish sepia or brown vinaceous.

Optimal media and cultivation conditions: On autoclaved banana leaves placed on SNA at 25 °C under near-ultraviolet light (12 h light, 12 h dark).

Distribution: Australia, China, Colombia, Italy and South Africa.

Hosts: Cissampelos capensis (Menispermaceae), Dactylis glomerata (Poaceae), Eucalyptus grandis (Myrtaceae), Leptocarpus sp. (Restionaceae), Lonicera sp. and Viburnum utile (Caprifoliaceae), and Spartium sp. (Fabaceae).

Disease symptoms: Leaf spots.

Notes: Wojnowiciella was established with W. eucalypti as type species, which differs from Septoriella hirta (syn. Wojnowicia hirta) by non-setous conidiomata, dark brown conidia and hyaline microconidia (Crous et al. 2015d). Although both genera belong to Phaeosphaeriaceae, Wojnowicia has been synonymised with Septoriella (Crous et al. 2015a). Currently Wojnowiciella comprises seven species isolated from leaf spots and twigs of different hosts (Table 21). Although they were associated with disease symptoms, their pathogenicity needs to be proven.

References: Wijayawardene et al. 2013 (morphology, as Wojnowicia); Crous et al., 2015d, Crous et al., 2016b (morphology); Li et al. 2015 (morphology and phylogeny, as Wojnowicia); Liu et al. 2015 (morphology and phylogeny, as Wojnowicia); Hernández-Restrepo et al. 2016c (morphology and phylogeny).

Authors: M. Hernández-Restrepo & P.W. Crous